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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
27/11/2008 |
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Data da última atualização: |
02/08/2019 |
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Autoria: |
RADAELLI, P.; FAJARDO, T. V. M.; NICKEL, O.; EIRAS, M.; PIO-RIBEIRO, G. |
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Afiliação: |
Paula Radaelli, Universidade Federal Rural de Pernambuco/Departamento de Agronomia; THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV; Marcelo Eiras, Instituto Biológico/Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal; Gilvan Pio-Ribeiro, Universidade Federal Rural de Pernambuco/Departamento de Agronomia. |
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Título: |
Production of polyclonal antisera using recombinant coat proteins of Grapevine leafroll-associated virus 2 and Grapevine virus B. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 10, p. 1405-1411, out. 2008. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Produção de anti-soros policlonais a partir de proteínas capsidiais recombinantes de Grapevine leafroll-associated virus 2 e Grapevine virus B. |
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Conteúdo: |
The objective of this work was to produce and characterize specific antisera against Brazilian isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine virus B (GVB), developed from expressed coat proteins (CPs) in Escherichia coli, and to test their possible use for the detection of these two viruses in diseased grapevines. The coat protein (CP) genes were RT-PCR-amplified, cloned and sequenced. The CP genes were subsequently subcloned, and the recombinant plasmids were used to transform E. coli cells and express the coat proteins. The recombinant coat proteins were purified, and their identities were confirmed by SDS-PAGE and Western blot and used for rabbit immunizations. Antisera raised against these proteins were able to recognize the corresponding recombinant proteins in Western blots and to detect GLRaV-2 and GVB in infected grapevine tissues, by indirect ELISA, discriminating healthy and infected grapevines with absorbances (A405) of 0.08/1.15 and 0.12/1.30, respectively. Expressing CP genes can yield high amount of viral protein with high antigenicity, and GLRaV-2 and GVB antisera obtained in this study can allow reliable virus disease diagnosis. |
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Palavras-Chave: |
ELISA indireto; GLRaV-2; GVB; indirect ELISA; proteína recombinante; recombinant protein; Western blot. |
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Thesaurus Nal: |
Vitis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE-2009-09/44983/1/43n10a20.pdf
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Marc: |
LEADER 02167naa a2200277 a 4500
001 1122230
005 2019-08-02
008 2008 bl uuuu u00u1 u #d
100 1 $aRADAELLI, P.
245 $aProduction of polyclonal antisera using recombinant coat proteins of Grapevine leafroll-associated virus 2 and Grapevine virus B.
260 $c2008
500 $aTítulo em português: Produção de anti-soros policlonais a partir de proteínas capsidiais recombinantes de Grapevine leafroll-associated virus 2 e Grapevine virus B.
520 $aThe objective of this work was to produce and characterize specific antisera against Brazilian isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine virus B (GVB), developed from expressed coat proteins (CPs) in Escherichia coli, and to test their possible use for the detection of these two viruses in diseased grapevines. The coat protein (CP) genes were RT-PCR-amplified, cloned and sequenced. The CP genes were subsequently subcloned, and the recombinant plasmids were used to transform E. coli cells and express the coat proteins. The recombinant coat proteins were purified, and their identities were confirmed by SDS-PAGE and Western blot and used for rabbit immunizations. Antisera raised against these proteins were able to recognize the corresponding recombinant proteins in Western blots and to detect GLRaV-2 and GVB in infected grapevine tissues, by indirect ELISA, discriminating healthy and infected grapevines with absorbances (A405) of 0.08/1.15 and 0.12/1.30, respectively. Expressing CP genes can yield high amount of viral protein with high antigenicity, and GLRaV-2 and GVB antisera obtained in this study can allow reliable virus disease diagnosis.
650 $aVitis
653 $aELISA indireto
653 $aGLRaV-2
653 $aGVB
653 $aindirect ELISA
653 $aproteína recombinante
653 $arecombinant protein
653 $aWestern blot
700 1 $aFAJARDO, T. V. M.
700 1 $aNICKEL, O.
700 1 $aEIRAS, M.
700 1 $aPIO-RIBEIRO, G.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 43, n. 10, p. 1405-1411, out. 2008.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 3 | |
| 1. |  | GAROFALO, D. F. T.; MESSIAS, C. G.; LIESENBERG, V.; BOLFE, E. L.; FERREIRA, C. Análise comparativa de classificadores digitais em imagens do Landsat-8 aplicados ao mapeamento temático. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 7, p. 593-604, jul. 2015. Título em inglês: Comparative analysis of digital classifiers of Landsat?8 images for thematic mapping procedures.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | GAROFALO, D. F. T.; MESSIAS, C. G.; LIESENBERG, V.; BOLFE, E. L.; FERREIRA, M. C. Análise comparativa de classificadores digitais em imagens do Landsat-8 aplicados ao mapeamento temático. Pesquisa Agropecuária Brasileira, Brasília, v. 50, n.7, p. 593-604, jul. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Territorial. |
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| 3. | | MESSIAS, C. G.; ALVES, H. M. R.; VOLPATO, M. M. L.; VIEIRA, T. G. C.; BORÉM, F. M.; BORÉM, R. A. T.; LACERDA, M. P. C. Mapping of coffee lands by remote sensing. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 223. p. 303| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 3 | |
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